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Anti-PIF4 Antibody (CAB20725)

SKU:
CAB20725
Product Type:
Antibody
Reactivity:
Plant - Arabidopsis
Host Species:
Rabbit
Isotype:
IgG
$215 - $563
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Description

Antibody Name:Anti-PIF4 Antibody (CAB20725)
Antibody SKU:CAB20725
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Arabidopsis thaliana
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300-400 of arabidopsis thaliana PIF4 (NP_565991.2).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Arabidopsis thaliana
Positive Samples:seedling, rosette leaf
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300-400 of arabidopsis thaliana PIF4 (NP_565991.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IDYL KSLQ LQLQ VMWM GSGM AAAA ASAP MMFP GVQP QQFI RQIQ SPVQ LPRF PVMD QSAI QNNP GLVC QNPV QNQI ISDR FARY IGGF PHMQ AATQ MQPM E
Cellular Location:
Calculated MW:48kDa
Observed MW:55KDa
Synonyms:AtPIF4, MFL8.13, MFL8_13, phytochrome interacting factor 4, SRL2
Background:Isolated as a semidominant mutation defective in red -light responses. Encodes a nuclear localized bHLH protein that interacts with active PhyB protein. Negatively regulates phyB mediated red light responses. Involved in shade avoidance response. Protein abundance is negatively regulated by PhyB.
Western blot analysis of extracts of various cell lines, using PIF4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Western blot analysis of extracts of various cell lines, using PIF4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

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