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Phospho-ULK1-S467 Rabbit Polyclonal Antibody (CABP1262)

SKU:
CABP1262
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
ATG1
ATG1A
UNC51
Unc51.1
hATG1
Research Area:
Autophagy
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-ULK1-S467 Rabbit Polyclonal Antibody
SKU:CABP1262
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around S467 of human ULK1 (NP_003556.2).
Sequence:SGST S
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:ATG1; ATG1A; UNC51; hATG1; Unc51.1; Phospho-ULK1-S467
Positive Sample:HeLa,Jurkat
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Preautophagosomal structure, cytosol.
Calculated MW:113kDa
Observed MW:140kDa

Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.

Purification Method:Affinity purification
Gene ID:8408
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Phospho-ULK1-S467 antibody (CABP1262) at 1:1000 dilution.HeLa and Jurkat cells were treated by Serum-starvation overnight at 37℃.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.

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