The Anti-phospho-SMAD3 (S423/S425) Antibody (CABP1263) is a crucial tool for researchers studying the phosphorylation of SMAD3 at serine residues 423 and 425. This antibody is raised in rabbits and has been validated for its reactivity with human samples, making it a reliable choice for Western blot applications.SMAD3 is a key signaling protein in the TGF-beta pathway, playing a critical role in regulating cell growth, differentiation, and immune responses. Phosphorylation of SMAD3 at specific sites, such as S423/S425, is known to modulate its activity and influence downstream signaling pathways. By targeting these phosphorylation sites, researchers can gain insights into the mechanisms underlying TGF-beta signaling and its implications in various physiological and pathological processes.
The Anti-phospho-SMAD3 (S423/S425) Antibody is an essential tool for investigating the roles of SMAD3 phosphorylation in cell signaling, gene expression, and disease progression. Its high specificity and sensitivity make it ideal for studies in areas such as immunology, cancer biology, and developmental biology, providing valuable information for the development of targeted therapies and diagnostic tools.
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.
Purification Method:
Affinity purification
Gene ID:
4088
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using (CABP1263) at 1:1000 dilution.Hela cells were treated by TGF-β (10 ng/ml) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
