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Phospho-RIPK1/RIP-S166 Rabbit Polyclonal Antibody (CABP1230)

SKU:
CABP1230
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
RIP
RIP-1
RIP1
RIPK1
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-RIPK1/RIP-S166 Rabbit Polyclonal Antibody
SKU:CABP1230
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around S166 of human RIPK1/RIP (NP_003795.2).
Sequence:MWSK L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:RIP; RIP1; AIEFL; IMD57; RIP-1; Phospho-RIPK1/RIP-S166
Positive Sample:HT-29+Z-VAD+TNF-α+SM-164
Conjugate:Unconjugated
Cellular Localization:cytosol, mitochondrion, plasma membrane, ripoptosome
Calculated MW:76kDa
Observed MW:75kDa

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth.

Purification Method:Affinity purification
Gene ID:8737
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of HT-29, using Phospho-RIPK1/RIP-S166 Rabbit pAb (CABP1230) at 1:800 dilution.HT-29 cells were treated with Z-VAD (50uM), human TNF-α (50ng/ml) and SM-164 (200nM) at 37℃ for 5 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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