The Anti-Phospho-RIPK1 (RIP S166) Antibody (CABP1230) is a valuable tool for researchers studying RIPK1, a key protein involved in cell death and inflammation pathways. This antibody, developed and validated for use in Western blot applications, specifically detects the phosphorylated form of RIPK1 at serine 166. RIPK1 is a crucial regulator of cell survival and death, and dysregulation of its activity has been implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. By targeting the phosphorylated form of RIPK1, researchers can gain insights into the intricate signaling pathways that govern cell fate decisions in these disease contexts.
The Anti-Phospho-RIPK1 (RIP S166) Antibody is highly specific and sensitive for detecting phosphorylated RIPK1 in human samples, making it a reliable tool for investigating the roles of RIPK1 in disease pathogenesis. Its use in immunology and cancer research can provide valuable information for the development of targeted therapies aimed at modulating RIPK1 activity for therapeutic benefit.
Product Name:
Phospho-RIPK1/RIP-S166 Rabbit Polyclonal Antibody
SKU:
CABP1230
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S166 of human RIPK1/RIP (NP_003795.2).
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth.
Purification Method:
Affinity purification
Gene ID:
8737
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of HT-29, using Phospho-RIPK1/RIP-S166 Rabbit pAb (CABP1230) at 1:800 dilution.HT-29 cells were treated with Z-VAD (50uM), human TNF-α (50ng/ml) and SM-164 (200nM) at 37℃ for 5 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
