The Anti-Phospho-RIP3 (S227) Antibody (CABP1257) is a valuable tool for researchers investigating the role of RIP3 in cell death pathways and inflammation. This antibody is raised in rabbits and specifically detects phosphorylation of RIP3 at serine 227. It is validated for use in Western blot applications and is highly reactive with human samples.RIP3, also known as receptor-interacting protein kinase 3, is a key player in necroptosis, a form of programmed cell death. Phosphorylation of RIP3 at serine 227 is known to regulate its activity and influence cell fate decisions. Understanding the signaling events involving RIP3 phosphorylation can provide insights into the mechanisms underlying cell death and inflammation in various disease states.
Research involving RIP3 and its phosphorylation at serine 227 has implications for therapeutic interventions in conditions such as cancer, neurodegenerative diseases, and inflammatory disorders. By targeting RIP3 and its phosphorylation status, researchers may be able to develop novel strategies for manipulating cell death pathways and modulating inflammatory responses. The Anti-Phospho-RIP3 (S227) Antibody (CABP1257) is a valuable tool for studying these processes and advancing our understanding of disease mechanisms.
Product Name:
Phospho-RIP3-S227 Polyclonal Antibody
SKU:
CABP1257
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S227 of human RIP3 (NP_006862.2).
Sequence:
EPSL V
Tested Applications:
IHC-PIF/ICCELISA
Recommended Dilution:
IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:
RIP3; Phospho-RIP3-S227
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, Mitochondrion, cytosol.
Calculated MW:
57kDa
Observed MW:
Refer to figures
The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.
Purification Method:
Affinity purification
Gene ID:
11035
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-RIP3-S227 Rabbit pAb (CABP1257) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
