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Phospho-PTEN-S380/T382/383 Polyclonal Antibody (CABP1231)

SKU:
CABP1231
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
10q23del
BZS
CWS1
DEC
GLM2
MHAM
MMAC1
PTEN1
TEP1
PTEN
PTENbeta
$587
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PTEN-S380/T382/383 Polyclonal Antibody
SKU:CABP1231
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S380 & T382 & T383 of human PTEN (NP_000305.3).
Sequence:YSDT TD
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta; Phospho-PTEN-S380/T382/383
Positive Sample:PC-12
Conjugate:Unconjugated
Cellular Localization:apical plasma membrane, cell projection, cytoplasm, cytoplasmic side of plasma membrane, cytosol, dendritic spine, extracellular region, myelin sheath adaxonal region, neuron projection, nucleoplasm, nucleus, plasma membrane, PML body, postsynaptic density
Calculated MW:47kDa
Observed MW:54kDa

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

Purification Method:Affinity purification
Gene ID:5728
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from PC-12 cells, using Phospho-PTEN-S380/T382/383 Rabbit pAb (CABP1231) at 1:1000 dilution.PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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