The Anti-Phospho-PTEN (S380/T382/383) Antibody (CABP1231) is a valuable tool for researchers studying PTEN, a tumor suppressor protein known for its role in regulating cell growth and division. This antibody, produced in rabbits, is highly specific to phosphorylated PTEN at sites S380, T382, and 383. It has been validated for use in Western blotting and other immunological techniques, making it an essential component in the study of cancer, cell signaling, and other related fields.PTEN is a critical regulator of cell survival and proliferation, functioning as a negative regulator of the PI3K/AKT signaling pathway. Phosphorylation of PTEN at specific sites can alter its activity and impact downstream signaling cascades.
By using the Anti-Phospho-PTEN (S380/T382/383) Antibody, researchers can investigate the phosphorylation status of PTEN in various cell types and gain insights into its functional implications in disease states such as cancer, neurodegeneration, and metabolic disorders.This antibody provides a reliable means of detecting and quantifying phospho-PTEN (S380/T382/383) levels, allowing for detailed analysis of PTEN-mediated signaling pathways. Its specificity and sensitivity make it a valuable asset for researchers interested in elucidating the molecular mechanisms underlying PTEN regulation and its potential as a therapeutic target.
Product Name:
Phospho-PTEN-S380/T382/383 Polyclonal Antibody
SKU:
CABP1231
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S380 & T382 & T383 of human PTEN (NP_000305.3).
apical plasma membrane, cell projection, cytoplasm, cytoplasmic side of plasma membrane, cytosol, dendritic spine, extracellular region, myelin sheath adaxonal region, neuron projection, nucleoplasm, nucleus, plasma membrane, PML body, postsynaptic density
Calculated MW:
47kDa
Observed MW:
54kDa
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5728
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from PC-12 cells, using Phospho-PTEN-S380/T382/383 Rabbit pAb (CABP1231) at 1:1000 dilution.PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
