The Anti-Phospho-POLR2A (S7) Antibody (CABP0969) is a valuable tool for researchers studying the phosphorylation of the POLR2A protein, a key component of the RNA polymerase II complex involved in transcription. This polyclonal antibody, raised in rabbits, is highly specific to the phosphorylated form of POLR2A at serine 7 in human samples.The antibody is validated for use in Western blot applications, enabling detection and analysis of phosphorylated POLR2A in various cell types.
This makes it ideal for studies in molecular biology, gene expression regulation, and transcriptional control.Understanding the mechanisms of POLR2A phosphorylation at serine 7 is essential for unraveling its role in transcriptional regulation and gene expression. The Anti-Phospho-POLR2A (S7) Antibody provides researchers with a reliable tool to investigate these processes and advance our understanding of transcriptional control in health and disease.
Product Name:
Phospho-POLR2A-S7 Rabbit Polyclonal Antibody
SKU:
CABP0969
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding S7 of human POLR2A CTD repeat YSPTSPS.
Sequence:
SPSY S
Tested Applications:
WBIHC-PChIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 ChIP,5μg antibody for 10μg-15μg of Chromatin
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Purification Method:
Affinity purification
Gene ID:
5430
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using Phospho-POLR2A CTD-S7 Rabbit pAb (CABP0969) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37℃ for 24 hours.MCF7 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
