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Phospho-PLN-S16/T17 Rabbit Polyclonal Antibody (CABP0956)

SKU:
CABP0956
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
CMD1P
CMH18
PLB
PLN
Research Area:
Cell Biology
$503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PLN-S16/T17 Rabbit Polyclonal Antibody
SKU:CABP0956
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S16 & T17 of human PLN (NP_002658.1).
Sequence:ASTI E
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:PLB; CMD1P; CMH18; Phospho-PLN-S16/T17
Positive Sample:HeLa,C6
Conjugate:Unconjugated
Cellular Localization:endoplasmic reticulum, mitochondrial membrane, mitochondrion, perinuclear region of cytoplasm
Calculated MW:6kDa
Observed MW:13kDa/17kDa

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.

Purification Method:Affinity purification
Gene ID:5350
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-PLN-S16/T17 Rabbit pAb (CABP0956) at 1:1000 dilution.Hela cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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