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Phospho-PLA2G4A-S505 Rabbit Polyclonal Antibody (CABP0968)

SKU:
CABP0968
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
PLA2G4A
PLA2G4
cPLA2
cPLA2-alpha
Research Area:
Metabolism
$503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PLA2G4A-S505 Rabbit Polyclonal Antibody
SKU:CABP0968
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S505 of human PLA2G4A (NP_077734.1).
Sequence:PLSP L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:GURDP; cPLA2; PLA2G4; cPLA2-alpha; Phospho-PLA2G4A-S505
Positive Sample:NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Cytoplasmic vesicle.
Calculated MW:85kDa
Observed MW:Refer to figures

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:5321
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Phospho-PLA2G4A-S505 Rabbit pAb (CABP0968) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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