The Anti-Phospho-PLA2G4A (S505) Antibody (CABP0968) is a valuable tool for researchers studying the phosphorylation of the PLA2G4A enzyme, which plays a crucial role in inflammation and immune response regulation. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in Western blot applications.Phosphorylation of PLA2G4A at the S505 site is known to regulate its enzymatic activity, affecting the production of inflammatory mediators and lipid signaling molecules. The Anti-Phospho-PLA2G4A (S505) Antibody enables researchers to detect and analyze phospho-S505 PLA2G4A levels in various cell types, providing insights into the mechanisms underlying inflammatory processes and immune regulation.
Studying the phosphorylation status of PLA2G4A can advance our understanding of diseases characterized by dysregulated inflammation, such as autoimmune disorders, cancer, and chronic inflammatory conditions. By targeting the phosphorylation of PLA2G4A, researchers can uncover potential therapeutic strategies for modulating immune responses and inflammatory pathways.
Product Name:
Phospho-PLA2G4A-S505 Rabbit Polyclonal Antibody
SKU:
CABP0968
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S505 of human PLA2G4A (NP_077734.1).
This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5321
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PLA2G4A-S505 Rabbit pAb (CABP0968) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
