The Anti-Phospho-PKR (EIF2AK2) T446 Antibody (CABP1251) is a powerful tool for researchers studying the phosphorylation of PKR (EIF2AK2) at threonine 446, a key event in the activation of this important regulatory protein. PKR is known for its role in the cellular response to viral infection, stress, and inflammation, and its phosphorylation at T446 is critical for its activity in modulating translation and immune response.This highly specific antibody, raised in rabbits, is validated for use in Western blot applications and has been shown to be highly reactive with human samples. By targeting the phosphorylated form of PKR at T446, researchers can gain insights into the signaling pathways and regulatory mechanisms involving this protein, particularly in the context of viral infections, autoimmune disorders, and cancer.
With its ability to detect and analyze phosphorylated PKR in various cell types, the Anti-Phospho-PKR (EIF2AK2) T446 Antibody (CABP1251) is an invaluable tool for studies in immunology, virology, and cancer research. By understanding the role of PKR phosphorylation at T446, researchers can uncover potential therapeutic targets and develop strategies for manipulating immune and stress response pathways in disease settings.
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.
Purification Method:
Affinity purification
Gene ID:
5610
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-PKR/EIF2AK2-T446 Rabbit pAb (CABP1251) at 1:2000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 180s.
