null

Phospho-PKR/EIF2AK2-T446 Rabbit Polyclonal Antibody (CABP1251)

SKU:
CABP1251
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
EIF2AK1
PKR
PPP1R83
PRKR
Research Area:
Immunology
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PKR/EIF2AK2-T446 Rabbit Polyclonal Antibody
SKU:CABP1251
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around T446 of human PKR/EIF2AK2 (NP_002750.1).
Sequence:KRTR SK
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:1000 - 1:5000 IF/ICC,1:50 - 1:200
Synonyms:PKR; PRKR; DYT33; LEUDEN; EIF2AK1; PPP1R83; Phospho-PKR/EIF2AK2-T446
Positive Sample:NIH/3T3+Calyculin A,NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus, perinuclear region.
Calculated MW:62kDa
Observed MW:68kDa/

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.

Purification Method:Affinity purification
Gene ID:5610
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells using Phospho-PKR/EIF2AK2-T446 Rabbit pAb (CABP1251) at 1:2000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 180s.

Related Products