The Anti-Phospho-PAK1/2/3 (S144/S141/S139) Antibody (CABP1158) is a highly specific antibody designed for research involving the phosphorylation of PAK1/2/3 at specific serine residues. PAK1/2/3 are a family of serine/threonine protein kinases that play key roles in cell motility, proliferation, and cytoskeletal remodeling. This polyclonal antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot applications. It specifically binds to the phosphorylated forms of PAK1/2/3 at serine 144, serine 141, and serine 139, allowing for the detection and analysis of these specific phosphorylation events in various cell types.
The phosphorylation of PAK1/2/3 at these residues has been associated with various cellular processes, including cell migration, invasion, and survival, making this antibody an invaluable tool for studies in cell biology, cancer research, and signaling pathways. Understanding the regulation of PAK1/2/3 phosphorylation at these sites is essential for unraveling the intricate mechanisms underlying cell signaling and cellular responses to external stimuli. Overall, the Anti-Phospho-PAK1/2/3 (S144/S141/S139) Antibody (CABP1158) is a critical research tool for investigating the functional role of
A synthetic phosphorylated peptide around S144 of human PAK1PAK1 (Q13153).
Sequence:
YMSF T
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
PAKalpha; Phospho-PAK1/2/3-S144/S141/S139
Positive Sample:
NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol, focal adhesion, microtubule organizing center, nuclear membrane, nucleoplasm, plasma membrane, ruffle membrane, Z disc
Calculated MW:
65kDa
Observed MW:
65kDa
This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]
Purification Method:
Affinity purification
Gene ID:
5058/5062/5063
Clone Number:
ARC1698
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb (CABP1158) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnightSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
