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Phospho-p53-T81 Rabbit Polyclonal Antibody (CABP1253)

SKU:
CABP1253
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
BCC7
LFS1
TRP53
TP53
BMFS5
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-p53-T81 Rabbit Polyclonal Antibody
SKU:CABP1253
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:A synthetic phosphorylated peptide around T81 of human TP53 (NP_000537.3).
Sequence:APTP A
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:P53; BCC7; LFS1; BMFS5; TRP53; Phospho-p53-T81
Positive Sample:C6
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body.
Calculated MW:44kDa
Observed MW:53kDa

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Purification Method:Affinity purification
Gene ID:7157
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of C6 cells, using Phospho-p53-T81 antibody (CABP1253) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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