The Anti-phospho-p53 (T81) Antibody (CABP1253) is a valuable tool for researchers studying the phosphorylation of p53 at threonine 81, a key post-translational modification that regulates the activity of the tumor suppressor protein p53. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Phosphorylation of p53 at threonine 81 has been linked to the activation of p53 in response to DNA damage and cellular stress, leading to the induction of cell cycle arrest, apoptosis, and DNA repair pathways.
Understanding the regulation of p53 phosphorylation at T81 is essential for unraveling the complex mechanisms that control cell fate decisions and tumor suppression.The Anti-phospho-p53 (T81) Antibody offers researchers a reliable tool for detecting and studying the phosphorylation status of p53 in various cell types and experimental conditions. Its applications in cancer research, DNA damage response studies, and drug development make it a valuable asset for investigations into the molecular pathways involved in tumorigenesis and therapeutic resistance.
Product Name:
Phospho-p53-T81 Rabbit Polyclonal Antibody
SKU:
CABP1253
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around T81 of human TP53 (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of C6 cells, using Phospho-p53-T81 antibody (CABP1253) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
