The Anti-Phospho-NF2 (S518) Antibody (CABP1225) offered by Assay Genie is a valuable tool for researchers studying NF2, a tumor suppressor protein implicated in the development of various types of cancers. This polyclonal antibody, produced in rabbits, is highly specific to the phosphorylated form of NF2 at serine 518 and is validated for use in immunohistochemistry and Western blot applications.NF2, also known as Merlin, plays a crucial role in regulating cell growth and proliferation, as well as cell-cell adhesion and communication. Phosphorylation of NF2 at serine 518 has been linked to its function in inhibiting cell proliferation and promoting apoptosis, making it a key target for cancer research.
The Anti-Phospho-NF2 (S518) Antibody allows for the detection and analysis of phosphorylated NF2 in various cell types, providing valuable insights into its role in tumorigenesis and potential therapeutic strategies targeting NF2.Researchers studying NF2-related cancers, such as schwannomas, meningiomas, and mesotheliomas, will benefit from the specificity and sensitivity of the Anti-Phospho-NF2 (S518) Antibody in their experiments. By understanding the phosphorylation status of NF2 and its impact on cell signaling pathways, researchers can advance our knowledge of cancer development and identify new targets for personalized treatment approaches.
Product Name:
Phospho-NF2-S518 Rabbit Polyclonal Antibody
SKU:
CABP1225
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S518 of human NF2 (NP_000259.1).
This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that link cytoskeletal components with proteins in the cell membrane. The encoded protein is involved in regulation of contact-dependent inhibition of cell proliferation and functions in cell-cell adhesion and transmembrane signaling. The encoded protein has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics, and proteins involved in regulating ion transport. Disruption of this protein's function has been implicated in tumorigenesis and metastasis. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities.
Purification Method:
Affinity purification
Gene ID:
4771
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-NF2-S518 Rabbit pAb (CABP1225) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
