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Phospho-NF2-S518 Rabbit Polyclonal Antibody (CABP1225)

SKU:
CABP1225
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
NF2
ACN
BANF
SCH
merlin
Research Area:
Cell Biology
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-NF2-S518 Rabbit Polyclonal Antibody
SKU:CABP1225
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S518 of human NF2 (NP_000259.1).
Sequence:RLSM E
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:ACN; SCH; BANF; merlin-1; Phospho-NF2-S518
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cell projection, Cytoplasm, Cytoplasmic granule, Cytoplasmic side, Nucleus, Nucleus, Peripheral membrane protein, cytoskeleton, filopodium membrane, perinuclear region, ruffle membrane.
Calculated MW:70kDa
Observed MW:70kDa

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that link cytoskeletal components with proteins in the cell membrane. The encoded protein is involved in regulation of contact-dependent inhibition of cell proliferation and functions in cell-cell adhesion and transmembrane signaling. The encoded protein has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics, and proteins involved in regulating ion transport. Disruption of this protein's function has been implicated in tumorigenesis and metastasis. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities.

Purification Method:Affinity purification
Gene ID:4771
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-NF2-S518 Rabbit pAb (CABP1225) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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