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Anti-Phospho-NCF4/p40-phox-T154 Antibody (CABP1193)

SKU:
CABP1193
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
NCF4
CGD3
NCF
P40PHOX
SH3PXD4
Research Area:
Cell Biology
€419
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Description

Anti-Phospho-NCF4/p40-phox-T154 Antibody (CABP1193)

The Anti-phospho-NCF4 (p40-phox) T154 Polyclonal Antibody (CABP1193) is a valuable tool for researchers studying the phosphorylation of NCF4, a key component of the phagocytic oxidase system. This antibody, raised in rabbits, is highly specific to the phosphorylated form of NCF4 at threonine 154 and has been validated for use in Western blot and immunohistochemistry applications.Phosphorylation of NCF4 at threonine 154 plays a crucial role in the activation of the phagocytic oxidase system, which is essential for the generation of reactive oxygen species and the clearance of pathogens by immune cells. By detecting and analyzing the phosphorylated form of NCF4, researchers can gain valuable insights into the regulation of this important immune response pathway.

This antibody is ideal for studies in immunology, infectious diseases, and inflammation, where understanding the phosphorylation status of NCF4 is essential for unraveling the complex mechanisms of the immune response. By using the Anti-phospho-NCF4 (p40-phox) T154 Polyclonal Antibody, researchers can further their understanding of immune regulation and potentially develop new therapies for diseases involving dysregulation of the immune system.

Product Name:Phospho-NCF4/p40-phox-T154 Rabbit pAb
Product Code:CABP1193
Size:50uL, 100uL
Synonyms:NCF4, CGD3, NCF, P40PHOX, SH3PXD4
Applications:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around T154 of human NCF4.
Applications:WB
Recommended Dilutions:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:Jurkat
Immunogen:A synthetic phosphorylated peptide around T154 of human NCF4.
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4689
Uniprot:Q15080
Cellular Location:Cytoplasm, Cytoplasmic side, Endosome membrane, Membrane, Peripheral membrane protein, cytosol
Calculated MW:39kDa
Observed MW:40KDa
UniProt Protein Function:p40phox: a component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst. Upon neutrophil stimulation, this protein and other cytosolic elements are sent to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. Responsible for the downregulation of NADPH-oxidase. Alternative splicing has been observed.
UniProt Protein Details:

Protein type:Oxidoreductase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytosol; endosome membrane; membrane; NADPH oxidase complex

Molecular Function:phosphatidylinositol 3-phosphate binding; protein binding; protein dimerization activity; superoxide-generating NADPH oxidase activator activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell redox homeostasis; immune response; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Iii

NCBI Summary:The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:Q15080
NCBI GenInfo Identifier:108884815
NCBI Gene ID:4689
NCBI Accession:Q15080.2
UniProt Secondary Accession:Q15080,O60808, Q86U56, Q9BU98, Q9NP45, A8K4F9,
UniProt Related Accession:Q15080
Molecular Weight:39,017 Da
NCBI Full Name:Neutrophil cytosol factor 4
NCBI Synonym Full Names:neutrophil cytosolic factor 4
NCBI Official Symbol:NCF4  
NCBI Official Synonym Symbols:NCF; CGD3; P40PHOX; SH3PXD4  
NCBI Protein Information:neutrophil cytosol factor 4
UniProt Protein Name:Neutrophil cytosol factor 4
UniProt Synonym Protein Names:Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40-phox; p40phox
Protein Family:Neutrophil cytosol factor
UniProt Gene Name:NCF4  
UniProt Entry Name:NCF4_HUMAN

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