The Anti-Phospho-MLKL (S441) Antibody (CABP1174) is a valuable tool for researchers investigating programmed cell death pathways, specifically necroptosis. This antibody targets the phosphorylated form of Mixed Lineage Kinase Domain-Like protein (MLKL) at Serine 441, a key site involved in cell death signaling.MLKL is a critical mediator of necroptosis, a form of programmed cell death that plays a role in inflammation, immune response, and tissue damage. Phosphorylation of MLKL at Serine 441 is a crucial step in the activation of necroptosis, making this antibody an important tool for studying the molecular mechanisms of cell death.The Anti-Phospho-MLKL (S441) Antibody is raised in rabbits and is highly specific for the phosphorylated form of MLKL at Serine 441.
It is validated for use in Western blot applications, allowing for the detection and analysis of phosphorylated MLKL in various cell types and tissues.Research into necroptosis and the role of MLKL in cell death pathways is vital for understanding disease processes such as cancer, neurodegenerative disorders, and inflammatory conditions. The Anti-Phospho-MLKL (S441) Antibody provides researchers with a powerful tool for investigating these critical pathways and developing potential therapeutic interventions targeting necroptosis.
Product Name:
Phospho-MLKL-S441 Rabbit pAb
Product Code:
CABP1174
Size:
20uL, 50uL, 100uL
Synonyms:
MLKL, hMLKL, mixed lineage kinase domain-like protein
Applications:
WB
Reactivity:
Human, Rat
Host Species:
Rabbit
Immunogen:
A phospho synthetic peptide corresponding to residues surrounding S441 of Mouse MLKL.
Applications:
WB
Recommended Dilutions:
WB 1:500 - 1:2000
Reactivity:
Human, Rat
Positive Samples:
C6
Immunogen:
A phospho synthetic peptide corresponding to residues surrounding S441 of Mouse MLKL.
Purification Method:
Affinity purification
Storage:
Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
Email for sequence
Gene ID:
74568
Uniprot:
Q9D2Y4
Cellular Location:
Cell membrane, Cytoplasm
Calculated MW:
53kDa/54kDa
Observed MW:
54KDa
UniProt Protein Function:
MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; TKL-Unique family Cellular Component: membrane; cytoplasm; plasma membrane Molecular Function: transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; protein kinase binding; ATP binding; protein kinase activity Biological Process: programmed cell death; protein amino acid phosphorylation
UniProt Protein Details:
NCBI Summary:
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
