The Anti-Phospho-MLKL (S358) Antibody (CABP1244) is a key tool for researchers studying programmed cell death pathways, specifically necroptosis. This antibody, derived from rabbits, is highly specific for detecting phosphorylated MLKL at serine 358, a crucial event in the necroptosis signaling cascade.With its high reactivity towards human samples, this antibody is a valuable tool for Western blot experiments, enabling researchers to study the activation of MLKL and its role in cell death processes. Its specificity for phospho-MLKL (S358) allows for precise detection and analysis in various cell types, making it an essential reagent for studies in cell biology, immunology, and disease research.
The phosphorylation of MLKL at serine 358 is a key event in necroptosis, a programmed cell death pathway with implications in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. Understanding the regulation and function of phospho-MLKL is crucial for developing targeted therapies that modulate cell death pathways in these pathological contexts.Overall, the Anti-Phospho-MLKL (S358) Antibody (CABP1244) is a valuable tool for researchers investigating necroptosis and its role in disease pathology, offering high specificity and sensitivity for detecting phosphorylated MLKL at serine 358 in human samples.
Product Name:
Phospho-MLKL-S358 Rabbit Polyclonal Antibody
SKU:
CABP1244
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S358 of human MLKL (NP_689862.1).
Sequence:
QTSM S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
hMLKL; Phospho-MLKL-S358
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm.
Calculated MW:
54kDa
Observed MW:
54kDa
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.
Purification Method:
Affinity purification
Gene ID:
197259
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of HeLa, using Phospho-MLKL-S358 antibody (CABP1244) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
