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Phospho-MLKL-S358 Rabbit Polyclonal Antibody (CABP1244)

SKU:
CABP1244
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
MLKL
hMLKL
mixed lineage kinase domain-like protein
Research Area:
Cell Death
$503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-MLKL-S358 Rabbit Polyclonal Antibody
SKU:CABP1244
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:A synthetic phosphorylated peptide around S358 of human MLKL (NP_689862.1).
Sequence:QTSM S
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:hMLKL; Phospho-MLKL-S358
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm.
Calculated MW:54kDa
Observed MW:54kDa

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.

Purification Method:Affinity purification
Gene ID:197259
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of HeLa, using Phospho-MLKL-S358 antibody (CABP1244) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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