The Anti-Phospho-MEK1 (T292) Antibody (CABP1221) is a valuable tool for researchers studying the phosphorylation of MEK1 at threonine 292. This antibody is raised in rabbits and is highly reactive with human samples, making it ideal for use in Western blot applications. By binding specifically to the phosphorylated form of MEK1 at threonine 292, this antibody enables the detection and analysis of MEK1 phosphorylation in various cell types.MEK1 is a critical kinase in the MAPK signaling pathway, playing a key role in cell proliferation, differentiation, and survival. Phosphorylation of MEK1 at threonine 292 is known to regulate its activity and downstream signaling, making it a crucial event in the regulation of cell growth and survival.
Understanding the regulation of MEK1 phosphorylation is important for studying various physiological and pathological processes, including cancer, as dysregulation of the MAPK pathway is commonly associated with tumorigenesis.Overall, the Anti-Phospho-MEK1 (T292) Antibody (CABP1221) is a valuable tool for researchers interested in studying the role of MEK1 phosphorylation in cell signaling and its implications in disease processes such as cancer. Its high reactivity and specificity make it a reliable choice for Western blot applications in various research settings.
Product Name:
Phospho-MEK1-T292 Rabbit Polyclonal Antibody
SKU:
CABP1221
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around T292 of human MEK1 (NP_002746.1).
Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body.
Calculated MW:
43kDa
Observed MW:
45kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method:
Affinity purification
Gene ID:
5604
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-T292 Rabbit pAb (CABP1221) at 1:1000 dilution.HeLa cells were treated by Nocodazole (100 ng/ml) at 37℃ for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
