The Anti-Phospho MCM2 (S139) Antibody (CABP1192) is a valuable tool for researchers studying cell cycle regulation and DNA replication. This polyclonal antibody, raised in rabbits, specifically targets the phosphorylated form of MCM2 at serine 139, a key marker of DNA replication initiation.With its high reactivity in human samples, this antibody is ideal for use in Western blot applications, enabling researchers to detect and analyze phosphorylated MCM2 in various cell types. By targeting this specific phosphorylation site, researchers can gain insights into the regulation of DNA replication and cell cycle progression.
The phosphorylation of MCM2 at serine 139 is crucial for the initiation of DNA replication, making this antibody essential for studies in fields such as cancer research, developmental biology, and cell biology. By understanding the role of phosphorylated MCM2 in these processes, researchers can uncover new therapeutic targets and potential treatment strategies for diseases related to cell proliferation and DNA replication dysfunction.
Product Name:
Phospho-MCM2-S139 Rabbit Polyclonal Antibody
SKU:
CABP1192
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S139 of human MCM2 (NP_004517.2).
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
Purification Method:
Affinity purification
Gene ID:
4171
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-MCM2-S139 Rabbit pAb (CABP1192) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.HeLa cells were treated by Serum-starvation overnight at 37℃.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
