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Phospho-MCM2-S139 Rabbit Polyclonal Antibody (CABP1192)

SKU:
CABP1192
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
MCM2
BM28
CCNL1
CDCL1
D3S3194
DFNA70
MITOTIN
cdc19
Research Area:
Cell Cycle
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-MCM2-S139 Rabbit Polyclonal Antibody
SKU:CABP1192
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S139 of human MCM2 (NP_004517.2).
Sequence:YDSD E
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN; Phospho-MCM2-S139
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:102kDa
Observed MW:125kDa

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.

Purification Method:Affinity purification
Gene ID:4171
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-MCM2-S139 Rabbit pAb (CABP1192) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.HeLa cells were treated by Serum-starvation overnight at 37℃.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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