Phospho-MAP3K5-S967 Rabbit Polyclonal Antibody (CABP1217)
- SKU:
- CABP1217
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- MAP3K5
- ASK1
- MAPKKK5
- MEKK5
- Research Area:
- Cell Death
Description
| Product Name: | Phospho-MAP3K5-S967 Rabbit Polyclonal Antibody |
| SKU: | CABP1217 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S967 of human MAP3K5 |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | ASK1; MEKK5; MAPKKK5; Phospho-MAP3K5-S967 |
| Positive Sample: | Jurkat |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Endoplasmic reticulum. |
| Calculated MW: | 155kDa |
| Observed MW: | 155kDa |
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
| Purification Method: | Affinity purification |
| Gene ID: | 4217 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Jurkat cells, using Phospho-MAP3K5-S967 Rabbit pAb (CABP1217) at 1:1000 dilution.Jurkat cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s. |
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