The Anti-Phospho-MAP3K5 (S967) polyclonal antibody (CABP1217) is specifically designed for research involving the phosphorylation of MAP3K5 at serine 967. MAP3K5, also known as apoptosis signal-regulating kinase 1 (ASK1), is a key regulator of cellular stress responses and apoptosis pathways. Phosphorylation at serine 967 is known to modulate the activity of MAP3K5, making this antibody a valuable tool for studying the signaling pathways involved in stress-induced apoptosis.Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By specifically targeting the phosphorylated form of MAP3K5 at serine 967, researchers can accurately detect and analyze the activation of this important signaling molecule in various cell types.
This makes the antibody ideal for studies in cell signaling, apoptosis, and stress response mechanisms.The phosphorylation of MAP3K5 at serine 967 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Understanding the regulation of MAP3K5 phosphorylation is crucial for unraveling the complex roles of this kinase in disease progression and identifying potential therapeutic targets. The Anti-Phospho-MAP3K5 (S967) antibody provides researchers with a reliable tool to investigate the functional significance of MAP3K5 phosphorylation and its impact on cellular processes involved in disease path
Product Name:
Phospho-MAP3K5-S967 Rabbit Polyclonal Antibody
SKU:
CABP1217
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding S967 of human MAP3K5
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
ASK1; MEKK5; MAPKKK5; Phospho-MAP3K5-S967
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Endoplasmic reticulum.
Calculated MW:
155kDa
Observed MW:
155kDa
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Purification Method:
Affinity purification
Gene ID:
4217
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-MAP3K5-S967 Rabbit pAb (CABP1217) at 1:1000 dilution.Jurkat cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
