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Phospho-LAT-Y191 Rabbit Polyclonal Antibody (CABP1188)

SKU:
CABP1188
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
LAT
LAT1
pp36
IMD52
Research Area:
Immunology
$503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-LAT-Y191 Rabbit Polyclonal Antibody
SKU:CABP1188
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around Y191 of human LAT (NP_001014987.1).
Sequence:REYV N
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:LAT1; pp36; IMD52; Phospho-LAT-Y191
Positive Sample:Jurkat,NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Single-pass type III membrane protein.
Calculated MW:28kDa
Observed MW:36kDa

The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.

Purification Method:Affinity purification
Gene ID:27040
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-LAT-Y191 Rabbit pAb (CABP1188) at 1:1000 dilution.Jurkat cells were treated by CD-3 (10 μg/mL) at 37℃ for 2 minutes after serum-starvation overnight.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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