The Anti-Phospho-LAT (Y191) Antibody (CABP1188) is a crucial tool for researchers studying immune signaling pathways and cellular responses. This polyclonal antibody, produced against phosphorylated LAT (linker for activation of T cells) at tyrosine 191, specifically detects this key protein modification in human samples. Validated for Western blot applications, this antibody enables precise detection and analysis of phospho-LAT in a variety of cell types.LAT is a critical component of T cell receptor signaling, playing a central role in the activation and proliferation of T cells. Phosphorylation of LAT at tyrosine 191 is a key event in this pathway, regulating downstream signaling cascades essential for immune responses.
By targeting this specific phosphorylation site, the Anti-Phospho-LAT (Y191) Antibody enables researchers to better understand the mechanisms underlying T cell activation and function.This antibody is invaluable for studies in immunology, cancer research, and autoimmune diseases where aberrant T cell signaling plays a significant role. By elucidating the role of phospho-LAT in these contexts, researchers can uncover potential therapeutic targets to modulate immune responses and develop novel treatments for immune-related disorders. The Anti-Phospho-LAT (Y191) Antibody is a powerful tool for unraveling the complexities of T cell signaling and advancing our knowledge of immune regulation.
Product Name:
Phospho-LAT-Y191 Rabbit Polyclonal Antibody
SKU:
CABP1188
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y191 of human LAT (NP_001014987.1).
Sequence:
REYV N
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
LAT1; pp36; IMD52; Phospho-LAT-Y191
Positive Sample:
Jurkat,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Single-pass type III membrane protein.
Calculated MW:
28kDa
Observed MW:
36kDa
The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
27040
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-LAT-Y191 Rabbit pAb (CABP1188) at 1:1000 dilution.Jurkat cells were treated by CD-3 (10 μg/mL) at 37℃ for 2 minutes after serum-starvation overnight.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
