The Anti-Phospho IRS1 (S636/639) Antibody (CABP0551) is a valuable tool for researchers studying insulin receptor substrate 1 (IRS1) phosphorylation events. This antibody, raised in rabbits, is highly specific for phosphorylation at serine 636/639 on IRS1 and is validated for use in Western blot applications. It allows for the detection and analysis of phosphorylated IRS1 in human samples, making it an essential component of studies in the fields of diabetes, metabolism, and signal transduction.IRS1 is a key mediator of insulin signaling, playing a crucial role in glucose metabolism and insulin sensitivity. Phosphorylation of IRS1 at serine 636/639 has been linked to insulin resistance and impaired insulin signaling, making it a relevant target for research into conditions such as type 2 diabetes and metabolic disorders.
Understanding the regulation of IRS1 phosphorylation is essential for developing strategies to improve insulin sensitivity and mitigate the negative effects of insulin resistance.Overall, the Anti-Phospho IRS1 (S636/639) Antibody (CABP0551) provides researchers with a powerful tool to investigate the role of IRS1 phosphorylation in insulin signaling and metabolic disorders, with the potential to advance our understanding of these complex biological processes and pave the way for the development of novel therapeutic interventions.
Product Name:
Phospho-IRS1-S636/639 Rabbit Polyclonal Antibody
SKU:
CABP0551
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S636 & S639 of human IRS1 (NP_005535.1).
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
Purification Method:
Affinity purification
Gene ID:
3667
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-IRS1-S636/639 Rabbit pAb (CABP0551) at 1:500 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
