The Anti-Phospho-IRS1 (S612) Antibody (CABP0553) is a valuable tool for researchers studying insulin receptor substrate 1 (IRS1) phosphorylation at serine 612. This phosphorylation event is critical for insulin signaling pathways and plays a key role in regulating glucose metabolism and energy homeostasis. This polyclonal antibody, generated in rabbits, is highly specific and sensitive for detecting phosphorylated IRS1 at serine 612 in human samples. The antibody has been validated for use in Western blot applications, allowing researchers to accurately measure levels of phospho-IRS1 in different cell types and tissues.Furthermore, understanding the phosphorylation status of IRS1 at serine 612 is important for investigating various diseases such as diabetes, obesity, and metabolic disorders.
Dysregulation of IRS1 phosphorylation can lead to insulin resistance and contribute to the development of these pathologies. By using this antibody, researchers can uncover novel insights into the role of phospho-IRS1 in these conditions and potentially identify new therapeutic targets for intervention.Overall, the Anti-Phospho-IRS1 (S612) Antibody (CABP0553) is a powerful tool for studying insulin signaling pathways and unraveling the intricate mechanisms that govern glucose homeostasis and metabolic health. Its high specificity and sensitivity make it a valuable asset for researchers in the fields of diabetes, metabolism, and endocrinology.
Product Name:
Phospho-IRS1-S612 Rabbit Polyclonal Antibody
SKU:
CABP0553
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S612 of human IRS1 (NP_005535.1).
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
Purification Method:
Affinity purification
Gene ID:
3667
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from C2C12 cells using Phospho-IRS1-S612 Rabbit pAb (CABP0553) at 1:1000 dilution. C2C12 cells were treated by Insulin (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
