Phospho-IRS1-S612 Rabbit Polyclonal Antibody (CABP0553)
- SKU:
- CABP0553
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- HIRS-1
- IRS1
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Product Name: | Phospho-IRS1-S612 Rabbit Polyclonal Antibody |
| SKU: | CABP0553 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic phosphorylated peptide around S612 of human IRS1 (NP_005535.1). |
| Sequence: | PMSP G |
| Tested Applications: | WB IHC-P ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 |
| Synonyms: | HIRS-1; Phospho-IRS1-S612 |
| Positive Sample: | C2C12,MCF7 |
| Conjugate: | Unconjugated |
| Cellular Localization: | caveola, cytoplasm, cytosol, nucleoplasm, nucleus, plasma membrane |
| Calculated MW: | 132kDa |
| Observed MW: | 180kDa |
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
| Purification Method: | Affinity purification |
| Gene ID: | 3667 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from C2C12 cells using Phospho-IRS1-S612 Rabbit pAb (CABP0553) at 1:1000 dilution. C2C12 cells were treated by Insulin (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
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