The Phospho-IP3R (S1756) Polyclonal Antibody (CABP1224) is a valuable tool for studying the phosphorylation of IP3R at serine 1756, a key event in calcium signaling pathways. This antibody, raised in rabbits, has been validated for use in various applications, including Western blot and immunohistochemistry.IP3R (Inositol 1,4,5-trisphosphate receptor) is a crucial protein involved in intracellular calcium release and signaling. Phosphorylation of IP3R at serine 1756 has been linked to regulation of calcium release from intracellular stores, impacting various cellular processes such as cell growth, differentiation, and apoptosis.
The Phospho-IP3R (S1756) Polyclonal Antibody enables specific detection and analysis of phosphorylated IP3R at serine 1756, providing researchers with a powerful tool for investigating the role of this phosphorylation event in various cellular functions. It is particularly useful for studies in cell signaling, neuroscience, and cancer research, where dysregulation of calcium signaling pathways can play a critical role in disease progression.
Product Name:
Phospho-IP3R-S1756 Polyclonal Antibody
SKU:
CABP1224
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S1756 of human ITPR1 (XP_011531986.1).
endoplasmic reticulum, nucleolus, perinuclear region of cytoplasm, Schaffer collateral - CA1 synapse
Calculated MW:
314kDa
Observed MW:
320kDa
This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
3708
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-IP3R-S1756 antibody (CABP1224) at 1:400 dilution.HeLa cells were treated by Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
