The Anti-Phospho IκBα (S36) Antibody (CABP0999) is a valuable tool for researchers studying the phosphorylation of IκBα at serine 36, a crucial event in the activation of the NF-κB signaling pathway. This antibody, generated in rabbits, specifically recognizes the phosphorylated form of IκBα at serine 36 in human samples and is validated for use in Western blot applications. By targeting this phosphorylation site, researchers can gain insight into the regulation of NF-κB signaling and its involvement in various physiological and pathological processes.The NF-κB signaling pathway is a key regulator of immune responses, inflammation, and cell survival, making it a focus of research in immunology, cancer biology, and inflammatory diseases.
Phosphorylation of IκBα at serine 36 is known to play a critical role in the activation of NF-κB and subsequent gene expression changes. By studying this phosphorylation event using the Anti-Phospho IκBα (S36) Antibody, researchers can better understand the molecular mechanisms underlying NF-κB activation and its implications for disease pathology.Overall, the Anti-Phospho IκBα (S36) Antibody (CABP0999) is a valuable tool for investigating the phosphorylation status of IκBα at serine 36 and its role in NF-κB signaling. Its high specificity
Product Name:
Phospho-IκBα-S36 Rabbit Monoclonal Antibody
SKU:
CABP0999
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S36 of human IκBα (P25963).
Sequence:
LDSM K
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:
IKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-S36
Positive Sample:
THP-1+LPS,THP-1+PMA,NIH/3T3+TNF-α,C6+TNF-α
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
36kDa
Observed MW:
39kDa
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
Purification Method:
Affinity purification
Gene ID:
4792
Clone Number:
ARC1543
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-IκBα-S36 Rabbit mAb (CABP0999) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight or treated by LPS (1 μg/mL) at 37℃ for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
