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Phospho-IκBα-S32 Rabbit Monoclonal Antibody (CABP0707)

SKU:
CABP0707
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Immunology
$503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-IκBα-S32 Rabbit Monoclonal Antibody
SKU:CABP0707
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S32 of human IKB alpha (P25963).
Sequence:HDSG L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:IKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-S32
Positive Sample:NIH/3T3+TNF-α
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:36kDa
Observed MW:39kDa

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Purification Method:Affinity purification
Gene ID:4792
Clone Number:ARC0147
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Phospho-IκBα-S32 Rabbit mAb (CABP0707) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 120s.

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