The Anti-phospho-IKBalpha (S32) Antibody (CABP0707) is a crucial tool for researchers studying the IKBalpha protein, a key regulator in the NF-kB signaling pathway involved in immune responses and inflammation. This highly specific antibody, produced in rabbits, is validated for use in Western blot applications and is particularly reactive with human samples.Phosphorylation of IKBalpha at S32 plays a critical role in the regulation of NF-kB activity, making this antibody essential for studies aiming to understand the intricacies of immune signaling and the development of targeted therapies for inflammatory disorders, autoimmune diseases, and cancer.
By facilitating the detection and analysis of phosphorylated IKBalpha, this antibody enables researchers to unravel the complex mechanisms underlying immune system dysfunction and explore novel treatment strategies.With its high specificity and sensitivity, the Anti-phospho-IKBalpha (S32) Antibody (CABP0707) is a valuable tool for immunologists and cancer researchers seeking to unravel the complexities of immune signaling pathways and develop innovative therapeutic interventions.
Product Name:
Phospho-IκBα-S32 Rabbit Monoclonal Antibody
SKU:
CABP0707
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S32 of human IKB alpha (P25963).
Sequence:
HDSG L
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
IKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-S32
Positive Sample:
NIH/3T3+TNF-α
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
36kDa
Observed MW:
39kDa
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
Purification Method:
Affinity purification
Gene ID:
4792
Clone Number:
ARC0147
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-IκBα-S32 Rabbit mAb (CABP0707) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 120s.
