The Anti-Phospho-HSF1 (S326) Antibody (CABP1189) is a valuable tool for researchers studying heat shock transcription factor 1 (HSF1) phosphorylation at serine 326. HSF1 is a key regulator of cellular stress response and has been implicated in various diseases, including cancer and neurodegenerative disorders. This polyclonal antibody, raised in rabbits, is highly specific to phosphorylated HSF1 at serine 326 and is validated for use in Western blot applications. It allows for the detection and analysis of phosphorylated HSF1 in human samples, making it an essential tool for studies in cancer biology, cell stress response, and protein regulation.
Understanding the phosphorylation status of HSF1 at serine 326 is crucial for determining its activity and function in cellular stress response pathways. This antibody provides researchers with a reliable and sensitive tool to investigate the role of phosphorylated HSF1 in various cellular processes, paving the way for potential therapeutic interventions targeting HSF1 in disease settings.
Product Name:
Phospho-HSF1-S326 Rabbit Polyclonal Antibody
SKU:
CABP1189
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S326 of human HSF1 (NP_005517.1).
Sequence:
LLSP T
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
HSTF1; Phospho-HSF1-S326
Positive Sample:
MCF7
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
57kDa
Observed MW:
66kDa
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.
Purification Method:
Affinity purification
Gene ID:
3297
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using Phospho-HSF1-S326 Rabbit pAb (CABP1189) at 1:500 dilution.MCF7 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
