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Phospho-HSF1-S326 Rabbit Polyclonal Antibody (CABP1189)

SKU:
CABP1189
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
HSTF1
Research Area:
Epigenetics and Nuclear Signaling
$503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-HSF1-S326 Rabbit Polyclonal Antibody
SKU:CABP1189
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S326 of human HSF1 (NP_005517.1).
Sequence:LLSP T
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:HSTF1; Phospho-HSF1-S326
Positive Sample:MCF7
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:57kDa
Observed MW:66kDa

The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.

Purification Method:Affinity purification
Gene ID:3297
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from MCF7 cells, using Phospho-HSF1-S326 Rabbit pAb (CABP1189) at 1:500 dilution.MCF7 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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