The Anti-phospho-Histone H3 (T32) Antibody (CABP0897) is a crucial tool for researchers studying cell division and chromatin remodeling processes. This antibody specifically targets phosphorylated Histone H3 at Threonine 32, a key modification associated with mitosis and chromosome condensation. Developed using advanced technology, this antibody offers high specificity and sensitivity for detecting phosphorylated Histone H3 at T32 in a variety of samples. It has been rigorously validated for use in techniques such as immunofluorescence, immunoprecipitation, and flow cytometry, providing reliable results for researchers investigating cell cycle progression, proliferation, and epigenetic modifications.
Histone H3 phosphorylation at T32 is known to play a crucial role in regulating gene expression during cell division and differentiation. Dysregulation of this process has been linked to various diseases, making this antibody a valuable tool for studying cancer, developmental biology, and stem cell research. By enabling precise detection and analysis of phosphorylated Histone H3 at T32, this antibody opens up new possibilities for understanding the molecular mechanisms involved in cellular processes.
Product Name:
Phospho-Histone H3-T32 Rabbit Polyclonal Antibody
SKU:
CABP0897
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic phosphorylated peptide around T32 of human Histone H3-T32 (NP_003520.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-Histone H3-T32 Rabbit pAb (CABP0897) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
