The Anti-Phospho-FOXO3A (S425) Antibody (CABP1200) is a versatile tool for researchers studying FOXO3A, a key transcription factor involved in regulating cellular processes such as cell cycle arrest, apoptosis, and oxidative stress response. This polyclonal antibody, produced in rabbits, specifically targets the phosphorylated form of FOXO3A at serine 425, a critical post-translational modification that regulates its activity.Validated for use in Western blot applications, the Anti-Phospho-FOXO3A (S425) Antibody provides high specificity and sensitivity for detecting phosphorylated FOXO3A in human samples.
By targeting this specific phosphorylation site, researchers can investigate the role of FOXO3A in various cellular pathways and disease processes, including cancer, neurodegenerative disorders, and aging.With its ability to selectively recognize phosphorylated FOXO3A, the Anti-Phospho-FOXO3A (S425) Antibody is an essential tool for unraveling the complex regulatory mechanisms that govern FOXO3A activity in health and disease. Researchers can use this antibody to deepen their understanding of FOXO3A signaling pathways and potentially identify new therapeutic targets for treating FOXO3A-related diseases.
Product Name:
Phospho-FOXO3A-S425 Rabbit Polyclonal Antibody
SKU:
CABP1200
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S425 of human FOXO3A (NP_001446.1).
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method:
Affinity purification
Gene ID:
2309
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from K-562 cells, using Phospho-FOXO3A-S425 Rabbit pAb (CABP1200) at 1:1000 dilution.K-562 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
