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Phospho-FOXO3A-S425 Rabbit Polyclonal Antibody (CABP1200)

SKU:
CABP1200
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
AF6q21
FKHRL1
FKHRL1P2
FOXO2
FOXO3A
Research Area:
Cell Death
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-FOXO3A-S425 Rabbit Polyclonal Antibody
SKU:CABP1200
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S425 of human FOXO3A (NP_001446.1).
Sequence:LGSP T
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2; Phospho-FOXO3A-S425
Positive Sample:K-562
Cellular Localization:cytoplasm, cytosol, mitochondrial matrix, mitochondrial outer membrane, nucleoplasm, nucleus
Calculated MW:71kDa
Observed MW:82kDa

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Purification Method:Affinity purification
Gene ID:2309
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from K-562 cells, using Phospho-FOXO3A-S425 Rabbit pAb (CABP1200) at 1:1000 dilution.K-562 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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