The Anti-Phospho EGFR (Y1173) Antibody (CABP1213) is a valuable tool for researchers studying epidermal growth factor receptor (EGFR) signaling pathways. This polyclonal antibody, produced in rabbits, is highly specific for the phosphorylated tyrosine 1173 residue on the EGFR protein. It has been validated for use in Western blot applications and is particularly reactive with human samples.EGFR is a key player in various cellular processes, including cell growth, proliferation, and survival. Phosphorylation of specific tyrosine residues, such as Y1173, is crucial for activating downstream signaling cascades that regulate these functions. The Anti-Phospho EGFR (Y1173) Antibody allows for the detection and analysis of EGFR phosphorylation levels in different cell types, providing valuable insights into EGFR-mediated signaling pathways.
Research involving EGFR signaling is important in understanding its role in cancer progression, as EGFR dysregulation is commonly associated with various cancer types. By studying the phosphorylation status of EGFR, researchers can gain a better understanding of how this receptor contributes to tumor growth and metastasis. The Anti-Phospho EGFR (Y1173) Antibody is a valuable tool for investigating the role of EGFR signaling in cancer and may lead to the development of targeted therapies for EGFR-overexpressing tumors.
Product Name:
Phospho-EGFR-Y1173 Rabbit Polyclonal Antibody
SKU:
CABP1213
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y1173 of human EGFR
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1173 Rabbit pAb (CABP1213) at 1:500 dilution.A-431 cells were treated by EGF (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
