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Phospho-EGFR-Y1173 Rabbit Polyclonal Antibody (CABP1213)

SKU:
CABP1213
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
ERBB
ERBB1
HER1
NISBD2
PIG61
mENA
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-EGFR-Y1173 Rabbit Polyclonal Antibody
SKU:CABP1213
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A phospho specific peptide corresponding to residues surrounding Y1173 of human EGFR
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; Phospho-EGFR-Y1173
Positive Sample:A-431
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:134kDa
Observed MW:175kDa

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

Purification Method:Affinity purification
Gene ID:1956
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1173 Rabbit pAb (CABP1213) at 1:500 dilution.A-431 cells were treated by EGF (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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