The Anti-Phospho-cGAS (S420) Antibody (CABP1228) is a valuable tool for researchers studying the cGAS protein, a key player in the cGAS-STING pathway that activates innate immune responses against viral and bacterial infections. This polyclonal antibody, raised in rabbits, is highly specific for detecting phosphorylation at serine 420 of the cGAS protein in human samples. Validated for use in Western blot applications, this antibody allows for precise detection and analysis of phospho-cGAS, providing insights into the regulation of innate immune signaling pathways.Phosphorylation of cGAS at serine 420 has been implicated in the activation of the cGAS-STING pathway, leading to the production of type I interferons and inflammatory cytokines in response to DNA damage or pathogen invasion.
Understanding the regulation of cGAS phosphorylation at this specific site is crucial for unraveling the molecular mechanisms underlying innate immune responses and developing novel therapeutic strategies targeting this pathway.This Anti-Phospho-cGAS (S420) Antibody is an essential tool for researchers in the fields of immunology, virology, and infectious diseases, offering unprecedented insights into the role of cGAS phosphorylation in innate immune signaling and immune defense mechanisms. Its high specificity and sensitivity make it a valuable asset for studying the intricate molecular interactions that govern immune responses and host-pathogen interactions.
Product Name:
Phospho-CGAS-S420 Rabbit Polyclonal Antibody
SKU:
CABP1228
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S420 of mouse CGAS (NP_775562.2).
Sequence:
FCSY H
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
Mb21d1; E330016A19Rik; Phospho-CGAS-S420
Positive Sample:
293T,C6
Conjugate:
Unconjugated
Cellular Localization:
cytosol, nucleoplasm, nucleus, plasma membrane
Calculated MW:
58kDa
Observed MW:
62kDa
The protein encoded by this gene is a DNA binding cytosolic protein that catalyzes the synthesis of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) after sensing the presence of DNA in the cytoplasm. cGAMP binds another protein, Stimulator of interferon genes (STING), leading to the induction of interferons, and a host immune response. Reduced expression of this gene inhibits interferon induction in the presence of some viral infections. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
214763
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-CGAS-S420 antibody (CABP1228) at 1:1000 dilution.293T and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
