The Anti-Phospho-cGAS (S291) Antibody (CABP1176) is a valuable tool for researchers studying cGAS, a key molecular sensor in the innate immune system that detects cytoplasmic DNA and triggers immune responses. This antibody, produced in rabbits, specifically targets the phosphorylated form of cGAS at serine 291, allowing for precise detection and analysis in various cell types and tissues. The phosphorylation of cGAS at serine 291 is known to regulate its activity and downstream signaling in response to viral infections and other pathological stimuli. By targeting this specific site, researchers can investigate the role of phosphorylated cGAS in immune responses, inflammation, and antiviral defense mechanisms.
Validated for use in Western blot applications, the Anti-Phospho-cGAS (S291) Antibody is a powerful tool for studying the complex regulatory mechanisms of cGAS activation and function. Its high reactivity with human samples makes it suitable for a wide range of research applications in immunology, virology, and cancer biology. Don't miss out on this innovative antibody for your next research project.
Product Name:
Phospho-CGAS-S291 Rabbit Polyclonal Antibody
SKU:
CABP1176
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S291 of mouse CGAS (NP_775562.2).
Sequence:
PGSP A
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
Mb21d1; E330016A19Rik; Phospho-CGAS-S291
Positive Sample:
293T
Cellular Localization:
cytosol, nucleoplasm, nucleus, plasma membrane
Calculated MW:
58kDa
Observed MW:
59kDa
The protein encoded by this gene is a DNA binding cytosolic protein that catalyzes the synthesis of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) after sensing the presence of DNA in the cytoplasm. cGAMP binds another protein, Stimulator of interferon genes (STING), leading to the induction of interferons, and a host immune response. Reduced expression of this gene inhibits interferon induction in the presence of some viral infections. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
214763
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-CGAS-S291 Rabbit pAb (CABP1176) at 1:500 dilution.293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
