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Phospho-Cdc6-S54 Rabbit Monoclonal Antibody (CABP1153)

SKU:
CABP1153
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
CDC18L
HsCDC18
HsCDC6
MGORS5
Research Area:
Cell Cycle
$503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Cdc6-S54 Rabbit Monoclonal Antibody
SKU:CABP1153
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse
Immunogen:A synthetic phosphorylated peptide around S54 of human Cdc6 (Q99741).
Sequence:PLSP R
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:CDC18L; HsCDC6; MGORS5; HsCDC18; Phospho-Cdc6-S54
Positive Sample:NIH/3T3 Paclitaxel,NIH/3T3 Hydroxyurea
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus
Calculated MW:63kDa
Observed MW:63kDa

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

Purification Method:Affinity purification
Gene ID:990
Clone Number:ARC1693
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of NIH/3T3 cells, using Phospho-Cdc6-S54 Rabbit mAb (CABP1153) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (1 uM) at 37℃ for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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