The Anti-Phospho-CDC6 (S54) Antibody (CABP1153) is a valuable tool for research involving CDC6, a key regulator of DNA replication and cell cycle progression. This polyclonal antibody, produced in rabbits, is highly specific and reactive with human samples, making it an ideal choice for Western blot applications. By targeting the phosphorylation site at serine 54 on the CDC6 protein, this antibody enables precise detection and analysis in various cell types.CDC6 is essential for the initiation of DNA replication and plays a crucial role in maintaining genomic stability. Phosphorylation of CDC6 at serine 54 is a critical regulatory mechanism that controls its activity during the cell cycle.
Research into CDC6 phosphorylation can provide valuable insights into the mechanisms of DNA replication and cell proliferation, with implications for cancer biology and other diseases.The Anti-Phospho-CDC6 (S54) Antibody is a powerful tool for studying the regulation of CDC6 and its impact on cellular processes. Its specificity and sensitivity make it a reliable option for researchers interested in unraveling the complexities of DNA replication and cell cycle control. By targeting the phosphorylated form of CDC6, this antibody opens up new possibilities for investigating the role of CDC6 in health and disease.
Product Name:
Phospho-Cdc6-S54 Rabbit Monoclonal Antibody
SKU:
CABP1153
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around S54 of human Cdc6 (Q99741).
Sequence:
PLSP R
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
CDC18L; HsCDC6; MGORS5; HsCDC18; Phospho-Cdc6-S54
Positive Sample:
NIH/3T3 Paclitaxel,NIH/3T3 Hydroxyurea
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus
Calculated MW:
63kDa
Observed MW:
63kDa
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
Purification Method:
Affinity purification
Gene ID:
990
Clone Number:
ARC1693
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Cdc6-S54 Rabbit mAb (CABP1153) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (1 uM) at 37℃ for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
