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Phospho-KIT-Y719 Rabbit Polyclonal Antibody (CABP0586)

SKU:
CABP0586
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
KIT
CD117
PBT
SCFR
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-KIT-Y719 Rabbit Polyclonal Antibody
SKU:CABP0586
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around Y721 of human CD117/c-Kit (NP_000213.1).
Sequence:TNEY M
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:PBT; SCFR; C-Kit; CD117; MASTC; Phospho-CD117/c-Kit-Y721
Positive Sample:NIH/3T3,
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Single-pass type I membrane protein.
Calculated MW:110kDa
Observed MW:120kDa/145kDa/

This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:3815
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Phospho-CD117/c-Kit-Y721 Rabbit pAb (CABP0586) at 1:500 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 20μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.

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