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Phospho-CARM1-S228 Rabbit Polyclonal Antibody (CABP0319)

SKU:
CABP0319
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
CARM1
PRMT4
$503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-CARM1-S228 Rabbit Polyclonal Antibody
SKU:CABP0319
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S228 of human PRMT4/CARM1 (NP_954592.1).
Sequence:VKSN N
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:PRMT4; Phospho-PRMT4/CARM1-S228
Positive Sample:Jurkat
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:66kDa
Observed MW:63kDa

This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9.

Purification Method:Affinity purification
Gene ID:10498
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells, using Phospho-PRMT4/CARM1-S228 Rabbit pAb (CABP0319) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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