The Anti-Phospho-CARM1 (S228) Antibody (CABP0319) is a valuable tool for researchers studying the phosphorylation of CARM1 at serine 228. CARM1, also known as coactivator-associated arginine methyltransferase 1, is a key regulator of gene expression and plays a crucial role in various cellular processes including transcriptional regulation, cell cycle progression, and tumorigenesis.This polyclonal antibody, generated in rabbits, is highly specific to the phosphorylated form of CARM1 at serine 228 and has been validated for use in Western blot applications. By targeting this specific phosphorylation site, researchers can study the activation and function of CARM1 in different cell types and contexts, particularly in the fields of cancer research, epigenetics, and gene regulation.
Understanding the role of phosphorylated CARM1 at serine 228 is essential for unraveling its impact on cellular signaling pathways and identifying potential therapeutic targets for diseases such as cancer. With its high reactivity and specificity, the Anti-Phospho-CARM1 (S228) Antibody (CABP0319) is an invaluable tool for investigating the complex mechanisms involving CARM1 phosphorylation and its implications in various biological processes.
Product Name:
Phospho-CARM1-S228 Rabbit Polyclonal Antibody
SKU:
CABP0319
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S228 of human PRMT4/CARM1 (NP_954592.1).
Sequence:
VKSN N
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
PRMT4; Phospho-PRMT4/CARM1-S228
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
66kDa
Observed MW:
63kDa
This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9.
Purification Method:
Affinity purification
Gene ID:
10498
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-PRMT4/CARM1-S228 Rabbit pAb (CABP0319) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
