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Phospho-c-Jun-T93 Rabbit Polyclonal Antibody (CABP1218)

SKU:
CABP1218
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
AP-1
AP1
c-Jun
JUN
c
Research Area:
Epigenetics and Nuclear Signaling
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-c-Jun-T93 Rabbit Polyclonal Antibody
SKU:CABP1218
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around T93 of human JUN (NP_002219.1).
Sequence:TPTP T
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-T93
Positive Sample:HeLa,NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:36kDa
Observed MW:48kDa

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Purification Method:Affinity purification
Gene ID:3725
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-c-Jun-T93 Rabbit pAb (CABP1218) at 1:500 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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