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Phospho-c-Jun-T91 Rabbit Polyclonal Antibody (CABP1216)

SKU:
CABP1216
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
AP-1
AP1
c-Jun
JUN
c
Research Area:
Epigenetics and Nuclear Signaling
€419
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Description

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Product Name:Phospho-c-Jun-T91 Rabbit Polyclonal Antibody
SKU:CABP1216
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T91 of human JUN (NP_002219.1).
Sequence:TTTP T
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-T91
Positive Sample:293T,NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:36kDa
Observed MW:36kDa

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Purification Method:Affinity purification
Gene ID:3725
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-c-Jun-T91 Rabbit pAb (CABP1216) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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