The Anti-Phospho c-Jun (T91) Antibody (CABP1216) is a highly specific tool for researchers studying the phosphorylation status of c-Jun, a key transcription factor that regulates gene expression in response to various stimuli. This polyclonal antibody, generated in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.Phosphorylation of c-Jun at Threonine 91 is known to play a crucial role in the activation of c-Jun, influencing its ability to regulate gene expression and participate in various cellular processes such as proliferation, differentiation, and apoptosis. The Anti-Phospho c-Jun (T91) Antibody (CABP1216) specifically binds to the phosphorylated form of c-Jun at Threonine 91, enabling researchers to detect and analyze its activity in different cell types.
By targeting the phosphorylation site of c-Jun, this antibody provides a valuable tool for studying signaling pathways and mechanisms involved in cellular responses to stimuli such as growth factors, stress, and inflammation. Understanding the role of phosphorylated c-Jun in these processes is essential for advancing research in fields such as oncology, neurobiology, and immunology.Overall, the Anti-Phospho c-Jun (T91) Antibody (CABP1216) offers researchers a reliable and effective means to investigate the function and regulation of c-Jun in cellular pathways, opening up new opportunities for exploring the implications of c-Jun
Product Name:
Phospho-c-Jun-T91 Rabbit Polyclonal Antibody
SKU:
CABP1216
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T91 of human JUN (NP_002219.1).
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Purification Method:
Affinity purification
Gene ID:
3725
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-c-Jun-T91 Rabbit pAb (CABP1216) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
