The Anti-Phospho c-Jun (S63) Antibody (CABP1190) is a valuable tool for studying cell signaling pathways involved in cancer and inflammatory processes. This antibody, raised in rabbits, is highly specific for detecting phosphorylated c-Jun protein at serine 63 in human samples. It has been validated for use in Western blot applications, allowing for accurate detection and analysis of phospho c-Jun levels in various cell types.The phosphorylation of c-Jun at serine 63 is known to play a key role in regulating gene expression and cell proliferation, making this antibody a critical tool for researchers studying the molecular mechanisms underlying cancer and inflammatory diseases.
By targeting this specific modification of c-Jun, researchers can gain valuable insights into the signaling pathways that contribute to disease progression and potential therapeutic targets.Overall, the Anti-Phospho c-Jun (S63) Antibody (CABP1190) is a reliable and specific tool for investigating signaling pathways involved in cancer and inflammation. Its high reactivity and specificity make it an ideal choice for researchers looking to explore the role of phosphorylated c-Jun in disease pathogenesis and potential therapeutic interventions.
Product Name:
Phospho-c-Jun-S63 Rabbit Polyclonal Antibody
SKU:
CABP1190
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S63 of human Jun (NP_002219.1).
Sequence:
LTSP D
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:
AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-S63
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
36kDa
Observed MW:
44kDa
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Purification Method:
Affinity purification
Gene ID:
3725
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-c-Jun-S63 Rabbit pAb (CABP1190) at 1:1000 dilution.293T cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
