The Anti-Phospho BRD4 (S1070) Antibody (CABP1181) is a valuable tool for researchers studying the phosphorylation of BRD4, a key regulator of gene expression and chromatin remodeling. This antibody, produced in rabbits, specifically detects phosphorylation at serine 1070 in BRD4 and is validated for use in Western blot and immunofluorescence applications.Phosphorylation of BRD4 at serine 1070 has been linked to its role in controlling transcription and cell cycle progression, highlighting its importance in various biological processes. By targeting this specific phosphorylation site, researchers can gain insight into the regulatory mechanisms involving BRD4 and its impact on cellular functions.
The Anti-Phospho BRD4 (S1070) Antibody is highly specific for human samples, allowing for precise detection and analysis of phosphorylated BRD4 in various cell types. This makes it an ideal tool for studies in molecular biology, cancer research, and drug development targeting BRD4-related pathways. By understanding the dynamics of BRD4 phosphorylation, researchers can uncover new therapeutic strategies for diseases linked to dysregulated gene expression and chromatin remodeling.
Product Name:
Phospho-BRD4-S1070 Rabbit Polyclonal Antibody
SKU:
CABP1181
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S1070 of human BRD4 (NP_490597.1).
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
23476
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-BRD4-S1070 Rabbit pAb (CABP1181) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
